Journal:

Article Title: Formation of Azole-Resistant Candida albicans by Mutation of Sterol 14-Demethylase P450

doi:

Figure Lengend Snippet: Polymorphisms observed around SRS-1 of C. albicans CYP51

Article Snippet: Lane M is the DNA size marker ( Bgl II- and Hin fI-digested pBR328). table ft1 table-wrap mode="anchored" t5 TABLE 4 caption a7 Template DNA Restriction site a Fluconazole MIC (μM) for the strain Hin dIII Afa I Xba I Cloned ATCC 90028-1 A A A Cloned ATCC 90028-2 P P P DUMC136 genomic P P P >200 S78941 genomic P P P >200 ATCC 90028 genomic P/A P/A P/A 1.6 CA1 genomic P/A P/A P/A 0.8 CA2 genomic A A P/A 0.4 CA3 genomic A A P/A 0.4 CA4 genomic P A P 0.8 CA5 genomic P A P 1.6 CA6 genomic A A P 26 CA7 genomic A A P 6.6 CA8 genomic A A P 3.3 CA9 genomic A A P 0.8 CA10 genomic A A A 1.6 Open in a separate window a The restriction sites of the 673-bp PCR products obtained from the indicated templates were analyzed as described in the legend to Fig. . P, present; A, absent; P/A, present on one allele and absent on another allele.

Techniques: Clone Assay

Journal:

Article Title: Formation of Azole-Resistant Candida albicans by Mutation of Sterol 14-Demethylase P450

doi:

Figure Lengend Snippet: MICs and IC 50 s of fluconazole for ergosterol biosynthesis in cell lysates of C. albicans ATCC 90028, DUMC136, and S78941

Article Snippet: Lane M is the DNA size marker ( Bgl II- and Hin fI-digested pBR328). table ft1 table-wrap mode="anchored" t5 TABLE 4 caption a7 Template DNA Restriction site a Fluconazole MIC (μM) for the strain Hin dIII Afa I Xba I Cloned ATCC 90028-1 A A A Cloned ATCC 90028-2 P P P DUMC136 genomic P P P >200 S78941 genomic P P P >200 ATCC 90028 genomic P/A P/A P/A 1.6 CA1 genomic P/A P/A P/A 0.8 CA2 genomic A A P/A 0.4 CA3 genomic A A P/A 0.4 CA4 genomic P A P 0.8 CA5 genomic P A P 1.6 CA6 genomic A A P 26 CA7 genomic A A P 6.6 CA8 genomic A A P 3.3 CA9 genomic A A P 0.8 CA10 genomic A A A 1.6 Open in a separate window a The restriction sites of the 673-bp PCR products obtained from the indicated templates were analyzed as described in the legend to Fig. . P, present; A, absent; P/A, present on one allele and absent on another allele.

Techniques:

Journal:

Article Title: Formation of Azole-Resistant Candida albicans by Mutation of Sterol 14-Demethylase P450

doi:

Figure Lengend Snippet: IC 50 s of fluconazole for the lanosterol 14-demethylase activity, lanosterol 14-demethylase activity, and P450 contents of the C. albicans ATCC 90028 and DUMC136 microsomes

Article Snippet: Lane M is the DNA size marker ( Bgl II- and Hin fI-digested pBR328). table ft1 table-wrap mode="anchored" t5 TABLE 4 caption a7 Template DNA Restriction site a Fluconazole MIC (μM) for the strain Hin dIII Afa I Xba I Cloned ATCC 90028-1 A A A Cloned ATCC 90028-2 P P P DUMC136 genomic P P P >200 S78941 genomic P P P >200 ATCC 90028 genomic P/A P/A P/A 1.6 CA1 genomic P/A P/A P/A 0.8 CA2 genomic A A P/A 0.4 CA3 genomic A A P/A 0.4 CA4 genomic P A P 0.8 CA5 genomic P A P 1.6 CA6 genomic A A P 26 CA7 genomic A A P 6.6 CA8 genomic A A P 3.3 CA9 genomic A A P 0.8 CA10 genomic A A A 1.6 Open in a separate window a The restriction sites of the 673-bp PCR products obtained from the indicated templates were analyzed as described in the legend to Fig. . P, present; A, absent; P/A, present on one allele and absent on another allele.

Techniques: Activity Assay

Journal:

Article Title: Formation of Azole-Resistant Candida albicans by Mutation of Sterol 14-Demethylase P450

doi:

Figure Lengend Snippet: The nucleotide (and deduced amino acid) sequence of the highly substituted region observed in CYP51 clones from wild-type C. albicans ATCC 90028 and fluconazole-resistant C. albicans isolates DUMC136 and S78941. The nucleotides and corresponding amino acids of the ATCC 90028-1 allele which are identical to those of the reported C. albicans CYP51 sequences (17) are fully indicated, and only substituted nucleotides and amino acids are shown for ATCC 90028, DUMC136, and S78941. The underlined region is one of the putative substrate recognition sites of CYP51, named SRS-1 (2). Dashes indicate amino acids identical to those of the ATCC 90028-1, and dots indicate nucleotides identical to those of the ATCC 90028-1 gene.

Article Snippet: Lane M is the DNA size marker ( Bgl II- and Hin fI-digested pBR328). table ft1 table-wrap mode="anchored" t5 TABLE 4 caption a7 Template DNA Restriction site a Fluconazole MIC (μM) for the strain Hin dIII Afa I Xba I Cloned ATCC 90028-1 A A A Cloned ATCC 90028-2 P P P DUMC136 genomic P P P >200 S78941 genomic P P P >200 ATCC 90028 genomic P/A P/A P/A 1.6 CA1 genomic P/A P/A P/A 0.8 CA2 genomic A A P/A 0.4 CA3 genomic A A P/A 0.4 CA4 genomic P A P 0.8 CA5 genomic P A P 1.6 CA6 genomic A A P 26 CA7 genomic A A P 6.6 CA8 genomic A A P 3.3 CA9 genomic A A P 0.8 CA10 genomic A A A 1.6 Open in a separate window a The restriction sites of the 673-bp PCR products obtained from the indicated templates were analyzed as described in the legend to Fig. . P, present; A, absent; P/A, present on one allele and absent on another allele.

Techniques: Sequencing, Clone Assay